This demo uses the standard scVelo pancreas subset derived from pancreatic epithelial and Ngn3-Venus fusion cells collected during the secondary transition of mouse development at embryonic day 15.5.
The data captures the transition from ductal and Ngn3-low/high endocrine progenitors through pre-endocrine cells into α, β, δ, and ε endocrine fates.
Bastidas-Ponce et al., “Comprehensive single cell mRNA profiling reveals a detailed roadmap for pancreatic endocrinogenesis,” Development (2019).